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Toraya, Seiko
  La curcumina inhibe la expresión de mediadores proinflamatorios y MMP-9 en células epiteliales gingivales estimuladas durante un período prolongado con lipopolisacáridos derivados de Porphyromonas gingivalis
  En: Odontology / The Society of the Nippon Dental University; odo, v.108 no 1. -- Vol. 108, no. 1 (January, 2020). -- Tokyo : Springer, 2001

  Curcumin, a yellow phytochemical found in the rhizomes of Curcuma longa, has various biological effects, including anti-oxidant and anti-inflammatory activities. In the present study, we examined the effect of curcumin on the expression of inflammatory cytokines in human gingival epithelial progenitor cells (HGEPs) stimulated for a prolonged period with lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. The cells were alternately cultured with LPS and/or curcumin every 3 days for 18 days. The expression levels of TNF-a, IL-1ß, IL-6, TIMP-1, and MMP-9 in the HGEPs were evaluated by quantitative real-time polymerase chain reaction. Enzyme-linked immunosorbent assay was used to measure the concentrations of these five proteins in the supernatant and nuclear factor (NF)-?B in the nuclear extracts. Curcumin inhibited the mRNA expression levels of TNF-a, IL-1ß, IL-6, and MMP-9 in HGEPs treated with curcumin over a prolonged period. Similarly, the expression levels of IL-1ß, IL-6, and MMP-9 were decreased in the culture supernatants. NF-?B activity was also inhibited in the cells cultured with curcumin. In conclusion, these findings indicate that curcumin inhibits the expression of inflammatory cytokines and MMP-9 in primary gingival epithelial cells stimulated with P. gingivalis-derived LPS via NF-?B activation.
  ISSN: 16181247

  1. PATOLOGIA BUCOMAXILOFACIAL; 2. CURCUMINA; 3. INHIBICION; 4. MEDIADORES PROINFLAMATORIOS; 5. LIPOPOLISACARIDOS; 6. CELULAS EPITELIALES; 7. PORPHYROMONAS GINGIVALIS; 8. ESTIMULACION I. Uehara, Osamu II. Hiraki, Daichi III. Harada, Fumiya IV. Neopane, Puja V. Morikawa, Tetsuro VI. Takai, Rie VII. Yoshida, Koki VIII. Matsuoka, Hirofumi IX. Kitaichi, Nobuyoshi X. Chiba, Itsuo XI. Abiko, Yoshihiro

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Toraya, Seiko
La curcumina inhibe la expresión de mediadores proinflamatorios y MMP-9 en células epiteliales gingivales estimuladas durante un período prolongado con lipopolisacáridos derivados de Porphyromonas gingivalis
En: Odontology / The Society of the Nippon Dental University; odo, v.108 no 1. -- Vol. 108, no. 1 (January, 2020). -- Tokyo : Springer, 2001

Curcumin, a yellow phytochemical found in the rhizomes of Curcuma longa, has various biological effects, including anti-oxidant and anti-inflammatory activities. In the present study, we examined the effect of curcumin on the expression of inflammatory cytokines in human gingival epithelial progenitor cells (HGEPs) stimulated for a prolonged period with lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. The cells were alternately cultured with LPS and/or curcumin every 3 days for 18 days. The expression levels of TNF-a, IL-1ß, IL-6, TIMP-1, and MMP-9 in the HGEPs were evaluated by quantitative real-time polymerase chain reaction. Enzyme-linked immunosorbent assay was used to measure the concentrations of these five proteins in the supernatant and nuclear factor (NF)-?B in the nuclear extracts. Curcumin inhibited the mRNA expression levels of TNF-a, IL-1ß, IL-6, and MMP-9 in HGEPs treated with curcumin over a prolonged period. Similarly, the expression levels of IL-1ß, IL-6, and MMP-9 were decreased in the culture supernatants. NF-?B activity was also inhibited in the cells cultured with curcumin. In conclusion, these findings indicate that curcumin inhibits the expression of inflammatory cytokines and MMP-9 in primary gingival epithelial cells stimulated with P. gingivalis-derived LPS via NF-?B activation.
ISSN: 16181247

1. PATOLOGIA BUCOMAXILOFACIAL; 2. CURCUMINA; 3. INHIBICION; 4. MEDIADORES PROINFLAMATORIOS; 5. LIPOPOLISACARIDOS; 6. CELULAS EPITELIALES; 7. PORPHYROMONAS GINGIVALIS; 8. ESTIMULACION I. Uehara, Osamu II. Hiraki, Daichi III. Harada, Fumiya IV. Neopane, Puja V. Morikawa, Tetsuro VI. Takai, Rie VII. Yoshida, Koki VIII. Matsuoka, Hirofumi IX. Kitaichi, Nobuyoshi X. Chiba, Itsuo XI. Abiko, Yoshihiro
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